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Structural characterization of an exopolysaccharide produced by Lactobacillus plantarum Ts isolated from Bulgarian wheat and rye flour

Tsveteslava Ignatova-Ivanova, Sevginar Ibryamova, Ismail Ismailov, Hasan Hasanov, Radoslav Ivanov

Abstract



An exopolysaccharide (EPS) was isolated from Lactobacillus plantarum Ts and purified by size exclusion chromatography train. L. plantarum Ts showed the highest production of EPS. The purified, homogeneous EPS by HPLS analisis had an average molecular weight of 9,03.104 Da The FTIR spectrum of the EPS from L. plantarum Ts showing the functional groups in the 4,000-400 cm-1 region. TLC analysis indicated that the EPS was a heteropolymer composed of fructose, and sucrose as monomeric constituent units. The strain L. plantarum Ts and pathogenic E. coli 3398, St. aureus 745, B. subtilis 6633, S. Typhi. 3591, L. monocytogenes 863 and E. aerogenes 3691 were tested for their growth utilizing the EPS from L. plantarum Ts as the sole carbon source for its possible use as a prebiotic. L. plantarum Ts exhibited growth in the EPS supplied medium compared with glucose as carbon source, whereas the pathogenic strains did not grow in the EPS-supplied medium. In vitro evaluations showed that, like other reported polysaccharides, this EPS displayed significant prebiotics properties.

Keywords


Exopolysaccharides, Lactobacillus plantarum Ts, prebiotic, lactic acid bacteria, pathogenic

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