Open Access Open Access  Restricted Access Subscription or Fee Access

Assessment of Pesticides Mixtures and Time–Dependent Responses of Toxicant

Nwokwu Gilbert Nwogboduhu

Abstract



The effects of Atrazine, Imidacloprid, and Nicotine mixtures were assessed using the aquatic crustacean, Artemia salina called the brine shrimp. The mixture of the insecticides was prepared in the ratios of 1:1, 1:10 and 10:1. The brine shrimp larvae were hatched in the laboratory of the Faculty of Science, Agriculture and Engineering of Newcastle University United Kingdom and the newly hatched larvae were exposed to the five concentrations of herbicide, insecticides mixtures, and their toxicity to brine shrimp larvae were determined at 6 and 24 hours. The dose-response of the brine shrimp larvae to the toxicants increased with increasing times of exposure and as the mortality increased the LD50 decreases until it levelled off. The acute toxicity for the insecticides was similar with the LD50s of Mixture of imidacloprid + atrazine ratio of 1:1 for 6 and 24h were 2.93709 and= 1.65632ppm, 1:10 for 6 and 24 h were 14.52 and 6.43ppm, 10:1 for 6 and 24 h were 3.43 and 1.52ppm. LD50 of Mixture of imidacloprid + nicotine ratio of 1:1 for 6 and 24 h were 2.64 and 2.17ppm, 1:10 for 6 and 24h were 3.72 and 3.09, 10:1 for 6 and 24h were 4.57 and 1.60. The least toxic was atrazine with LD50 for 6 and 24h were 17.12 and 3.14ppm respectively. The doses of the pesticides mixtures increased mortality of the brine shrimp with time of exposure. The time required to reach the plateau when shrimp are expected to have died was at 24 hours.

Keywords


Assessment, pesticides, toxicity, Time

Full Text:

PDF


Disclaimer/Regarding indexing issue:

We have provided the online access of all issues and papers to the indexing agencies (as given on journal web site). It’s depend on indexing agencies when, how and what manner they can index or not. Hence, we like to inform that on the basis of earlier indexing, we can’t predict the today or future indexing policy of third party (i.e. indexing agencies) as they have right to discontinue any journal at any time without prior information to the journal. So, please neither sends any question nor expects any answer from us on the behalf of third party i.e. indexing agencies.Hence, we will not issue any certificate or letter for indexing issue. Our role is just to provide the online access to them. So we do properly this and one can visit indexing agencies website to get the authentic information.