Open Access Open Access  Restricted Access Subscription or Fee Access

Recombinant Allergens in Diagnostics of Birch Pollen Allergy and Grass Pollen Allergy

Carmen de Matos, Ralf Bauer

Abstract


Current serologic diagnosis of birch pollen allergy and timothy pollen allergy is mainly performed with natural pollen extract. We assume that a more exact and efficient diagnostics and therapy might be achieved by using one of the recombinant allergens rBet v 1 or rBet v 2 respectively rPhl p 1, rPhl p 2 or rPhl p 5. Therefore IgE concentrations of rBet v 1 and rBet v 2 were compared to IgE concentrations of natural birch pollen allergen using the CAP-FEIA as well as IgE concentrations of rPhl p 1, rPhl p 2 and rPhl p 5 were compared to those of natural timothy pollen extract. Sera of 165 patients from the allergy outpatients clinic of the University of Bonn were included into the analysis. By calculating the rank correlation, autocorrelation, linear and multiple regression and Student´s T-Test rBet v 1 was confirmed being a major allergen of birch as well as rBet v 1 being an indicator for diagnostic of birch pollen allergy and specific immune therapy. Concerning timothy grass, the three recombinant allergens rPhl p 1, rPhl p 2 and rPhl p 5 can replace natural timothy pollen extract highly significantly.

Keywords


recombinant allergens, rBet v 1, rBet v 2, rPhl p 1, rPhl p 2, rPhl p 5, birch pollen allergy, grass pollen allergy, allergogramm, correlation, autocorrelation, regression, Student´s T-Test

Full Text:

PDF


Disclaimer/Regarding indexing issue:

We have provided the online access of all issues and papers to the indexing agencies (as given on journal web site). It’s depend on indexing agencies when, how and what manner they can index or not. Hence, we like to inform that on the basis of earlier indexing, we can’t predict the today or future indexing policy of third party (i.e. indexing agencies) as they have right to discontinue any journal at any time without prior information to the journal. So, please neither sends any question nor expects any answer from us on the behalf of third party i.e. indexing agencies.Hence, we will not issue any certificate or letter for indexing issue. Our role is just to provide the online access to them. So we do properly this and one can visit indexing agencies website to get the authentic information. Also: DOI is paid service which provided by a third party. We never mentioned that we go for this for our any journal. However, journal have no objection if author go directly for this paid DOI service.